sham operated mice  (Miltenyi Biotec)

Journal: bioRxiv

Article Title: Genomic Analysis of Progenitors in Viral Infection Implicates Glucocorticoids as Suppressors of Plasmacytoid Dendritic Cell Generation

doi: 10.1101/2024.10.28.620771

Figure Lengend Snippet: (A) Conceptual and non-proportional diagram of the continuous model of DC hematopoiesis. Lin − c-kit int/lo Flt3 + progenitors are outlined in red. Classically defined progenitor populations (CD115+ CDP (CDP M+ ), CD115-CDP (CDP M- ), CLP, black boxes) partially overlap in potential. (B) Lin − c-kit int/lo Flt3 + progenitors were isolated from CD45.2 + mice infected with LCMV Cl13 at day 8 p.i., or mice that were left uninfected (Un) and transferred into infection matched or Un controls, respectively. Eight days after transfer, spleens were harvested, and DC development assessed by flow cytometry. (C-H) C57BL6/J mice were infected with LCMV ARM or Cl13 or left uninfected (Un), sacrificed at day 8 p.i., and Lin − c-kit int/lo Flt3 + progenitors were FACS-purified from BM for RNA-seq (D-E) and ATAC-seq (F-H) analyses. Data are representative of three independent repeats, each with 3-5 mice pooled per group. (D) Hierarchical clustering of RNA-seq profiles on the whole transcriptome by using Pearson correlation. (E) Venn diagram showing overlap between differentially expressed genes (DEGs) from Cl13 vs. Un and ARM vs. Un comparisons. (F) Hierarchical clustering of ATAC-seq profiles by using Pearson correlation. (G) Venn diagram showing overlap between differentially accessible (DA) chromatin regions from Cl13 vs. Un and ARM vs. Un comparisons. (H) Number of differential peaks that opened (left, red; right, green) or closed (blue) during infection. Graphs depict means ± SEM and symbols represent individual mice. (B) Data are pooled from two independent experiments with 2– 4 mice/group. (D-H) Data are representative of three independent repeats, each with 3-5 mice pooled per group. * p < 0.05, ****p < 0.0001. Statistical significance was determined by unpaired student’s t-test (B).

Article Snippet: C57BL6/J mice, CD45.1 + mice, sham-operated and ADX mice (7-8 weeks old) were purchased from The Jackson Laboratory.

Techniques: Isolation, Infection, Flow Cytometry, Purification, RNA Sequencing Assay

Journal: Scientific Data

Article Title: Murine femur micro-computed tomography and biomechanical datasets for an ovariectomy-induced osteoporosis model

doi: 10.1038/s41597-021-01012-8

Figure Lengend Snippet: Experimental workflow. Both femurs from C57BL/6JOlaHsd OVX and sham female mice were extracted from each animal; one femur was used for micro-CT measurements and the other for biomechanical testing.

Article Snippet: Briefly, C57BL/6JOlaHsd ovariectomized (OVX) and sham operated (sham) female mice at 8-weeks of age were purchased from ENVIGO.

Techniques: Micro-CT

Journal: bioRxiv

Article Title: Remdesivir inhibits renal fibrosis in obstructed kidneys

doi: 10.1101/2020.04.01.019943

Figure Lengend Snippet: Intraperitoneal (i.p.) administration of remdesivir inhibited fibrosis in UUO mice. After sham or UUO operation, wide type c57 mice were treated with 10 mg/kg/d remdesivir by i.p. injection for 10 days. Serum and kidney tissue were collected 1 hour after remdesivir injection at day 10. (A) Renal fibrosis was assessed by Masson’s trichrome staining. (B) The expression of FN, collagen I (Col-1), pSmad3, and α-SMA by Western blotting. One representative of at least three independent experiments is shown. (C) Liver function (ALT and AST) and renal function (Scr and BUN) were assessed. (D) The concentrations of nucleoside metabolite (GS-441524) and alanine metabolite (Ala-Met), two remdesivir metabolites, in serum and kidneys were determined by LC-MS/MS. Data represent mean ± SD. ND represents not determined. * P < 0.05 versus Sham-vehicle; ** P < 0.01 versus Sham-vehicle; # P < 0.05 versus UUO-vehicle; ## P < 0.01 versus UUO-vehicle.

Article Snippet: The protein expression of FN, collagen-I (Col-I), pSmad3, and α-SMA were up-regulated in UUO mouse kidneys as compared with that in sham operated mouse kidneys, and the treatment with remdesivir significantly reduced the expression of these pro-fibrotic proteins in UUO mouse kidneys ( ).

Techniques: Injection, Staining, Expressing, Western Blot, Liquid Chromatography with Mass Spectroscopy